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KMID : 0369319970170020141
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Allergy
1997 Volume.17 No. 2 p.141 ~ p.150
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House dust mite dose not directly activate the human peripheral blood eosinophils in house dust mite-sensitized asthmatics
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Kim Mi-Kyeong
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Abstract
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In asthma, eosinophil roles as main end inflammatory effector cell, but neither the activation process nor the stimuli of that is known clearly, yet. Even though allergic asthma is IgE-mediated type I hypersensitivity disease, it is not clear whether there is FcεRI on the surface of eosinophil like mast cell or basophil which is activated through the FcεRl bridging with sensitized allergens. In this study, we speculate if there’s FcεRI on the eosinophil, sensitized allergen activates it like mast cell in allergic asthmatics, and with this result we can find out one of the stimuli of eosinophil in asthma and can suggest the possibility of the existence of FcεRI on the eosinophil. We did direct stimulation of the house dust mite-sensitized astknatics’purified peripheral blood normodense eoisnophils with Dermatophagoides pteronyssiuns. Then we measured the LTC4 and ECP from the supernatant by RIA kit and observed the morphological changes with electron microscopy. There was neither increase in LTC4 and ECP level nor the morphological changes in asthmatics. And there were no significant differences in both LTC4, and ECP level, and morphologic changes between asthmatics and controls. We would summerize that house dust mite dose not dirctly activate eosinophil
from house dust mite-sensitized asthmatics and couldn’t find out the possibility that there’s FcεRI on the surface of eoslnophils. But no exitstence of FcεRI on the eosinophil is the indirect evidence, so from now on we try to find more direct evidence of that by blotting.
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KEYWORD
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asthma, eosinophil activation, house dust mite,
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